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At 12 hpi, cells were lysed by resuspending in 3 mL of mild lysis buffer (10 mM Tris, pH 7.4, 150 mM NaCl, 2 mM MgCl 2 1 mM EDTA, 1 mM DDT and 0.5 % Igepal) and incubated on ice for 10 min, followed by vortexing for 30 s

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Production of TDE0362 polyclonal and monoclonal antibodies The above purified F0362 protein was used to generate polyclonal antibodies in rats on a fee-for-service basis in General Bioscience (Brisbane, California) as previously described (Kurniyati et al, 2022a)

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