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Behavioral testing was followed by biochemical analyses on colon, faces, serum, cerebellum, and prefrontal cortex, assessing tryptophan metabolites, serotonin (5-HT) pathway intermediates, neurotransmitters (GABA, glutamate, dopamine, acetylcholine) via UPLC, UPLC-TQD, and ELISA, alongside gut microbiota profiling through 16S r RNA sequencing

Relationship of resting metabolic rate to body composition and protein turnover

This approach involved iteratively removing one study at a time and recalculating the pooled effect estimates

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doi: 10.1371/journal.pone.0154654 12

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