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Semin Thorac Cardiovasc Surg 33(4):1158-1168, 2021

Cells were resuspended in lysis buffer (50 mM sodium phosphate, pH 7.5, 500 mM NaCl, 0.5 mM TCEP, 5% glycerol, 1.0% Triton X-100, 0.1 mM PLP, 2 mg/ml lysozyme) and lysed by sonication

Given that isoelectric focusing necessitates the free migration of proteins based on their charge properties within an electric field, lower concentrations of polyacrylamide gels (e.g., 4%) are typically used to prevent molecular sieving

Phytomedicine 118 , 154919

Mechanistically, high expression of BCAT1 reduces the NAD + /NADH ratio, increases mTORC1 activity, and promotes oxidative phosphorylation and nucleotide biosynthesis

Mehta P, McAuley DF, Brown M, Sanchez E, Tattersall RS, Manson JJ, et al