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Each method has its pros and cons

Bethanechol chloride does not cross the blood-brain barrier because of its charged quaternary amine moiety

I.BrivioP.MiddelmanA.BaderM.FumagalliF.et al

Schmidt ME, Scherer S, Wiskemann J, Steindorf K

Nrf2 improves endothelial function by resisting oxidative stress and mitochondrial damage, thereby delaying atherosclerosis (245)

Neurons were visualized with infrared differential interference contrast imaging and a CCD camera under a water-immersion objective (40 , 0.8 numerical aperture)