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Peptide separation was subsequently performed at 50 C and a flow rate of 300 nl min 1 on a 50 m 25 cm separation column (Acclaim PepMap RSLC, Dionex), applying a gradient ranging from 2.4 to 32.0% acetonitrile over the course of 90 min

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A 750 mg single 3-hour infusion has been reported to raise blood NAD+ ~398% over baseline in published pilot work

This boosts energy levels, enhances stamina, supports overall physical performance, and helps maintain vitality, making it an ideal therapy for fatigue, low energy, and active lifestyles