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In this case, overall assessment (WoE) of the general biological plausibility and of the empirical support of KERs was considered as high for this AOP, as well as essentiality, once for this criterion the first four KEs that trigger the AO are also classified as such

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Thermal shift assay (TSA) Samples were prepared by using a final concentration of 0.25 mg/ml TGR diluted in TGR reaction buffer to the desired volume