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Subsequently, the mesh insert carrying the slices was transferred into higher concentrations of the vitrification solution, which was set to 10 C in a second polystyrene box containing a 65% w/v ethylene glycol in water bath (kept at 10 C using dry ice)

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Reconstitution & Storage Guidelines Reconstitution Protocol Allow vial to reach room temperature before opening to prevent condensation Add sterile water or 0.1% acetic acid slowly down the vial wall Swirl gently avoid vigorous shaking Typical working concentration: 15 mg/mL depending on experimental protocol Filter through 0.22m sterile filter if required Storage Requirements Unopened powder: 20C, stable for 24+ months Reconstituted solution: 28C, use within 24 weeks Long-term storage: Aliquot and freeze at 80C to avoid freeze-thaw cycles Protect from light and moisture throughout MOTS-c Peptide UK FAQ What is MOTS-c peptide