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After administration, peptides are generally broken down by enzymes called peptidases into smaller amino acid fragments

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Local and Theoretical Concerns Local reactions observed or theoretically expected in animal models include: Transient erythema at topical application sites Mild irritation or inflammatory responses at injection sites Potential for altered MMP activity with excessive dosing Theoretical risk of excessive collagen degradation if matrix enzyme balance is disrupted The concern about copper toxicityvalid for free copper ionsappears mitigated by the peptides chelation mechanism

This process increases the water-air interface and allows extra heat to evaporate at a fast pace

doi: 10.1016/j.joca.2013.05.013

After storage at 20 C overnight, diluted samples were thawed again, centrifuged for 10 min at 20,000 g and 4 C